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KMID : 0545120160260122060
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 12 p.2060 ~ p.2065
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Virulence Factors and Stability of Coliphages Specific to Escherichia coli O157:H7 and to Various E. coli Infection
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Kim Eun-Jin
Chang Hyun-Joo
Kwak Soo-Jin
Park Jong-Hyun
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Abstract
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Characteristics of E. coli O157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E. coli serotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factors stx1, stx2, ehxA, and saa between the two groups were not differentiated. Broad-host-range coliphages showed lower stability at 70oC, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerant E. coli O157:H7.
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KEYWORD
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E.coli O157:H7, host spectrum, bacteriophage, virulence gene, stability
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